Abstract PROBLEM TO BE SOLVED: To provide a useful observation method of cuticle cells, in evaluating or screening cosmetics, skin external preparations, or the like using three-dimensional cultured skin model.SOLUTION: The observation method of cuticle cells includes removing the top surface of an epithelium reconstructed body and observing the epithelium reconstructed body with a microscope. Preferably, the top surface of the epithelium reconstructed body is removed by tape-stripping, and a confocal laser microscope is used as the microscope.SELECTED DRAWING: Figure 7 【課題】3次元培養皮膚モデルを用いた化粧料や皮膚外用剤等の評価やスクリーニングを行う際、より有用な角質細胞の観察方法を提供すること。【解決手段】上皮再構築体の上面を除去し、上皮再構築体を顕微鏡で観察することを含む、角質細胞の観察方法である。好ましくは、前記上皮再構築体の上面の除去はテープストリッピングで行い、顕微鏡は共焦点レーザー顕微鏡を用いる。【選択図】図7
AbstractList PROBLEM TO BE SOLVED: To provide a useful observation method of cuticle cells, in evaluating or screening cosmetics, skin external preparations, or the like using three-dimensional cultured skin model.SOLUTION: The observation method of cuticle cells includes removing the top surface of an epithelium reconstructed body and observing the epithelium reconstructed body with a microscope. Preferably, the top surface of the epithelium reconstructed body is removed by tape-stripping, and a confocal laser microscope is used as the microscope.SELECTED DRAWING: Figure 7 【課題】3次元培養皮膚モデルを用いた化粧料や皮膚外用剤等の評価やスクリーニングを行う際、より有用な角質細胞の観察方法を提供すること。【解決手段】上皮再構築体の上面を除去し、上皮再構築体を顕微鏡で観察することを含む、角質細胞の観察方法である。好ましくは、前記上皮再構築体の上面の除去はテープストリッピングで行い、顕微鏡は共焦点レーザー顕微鏡を用いる。【選択図】図7
Author ATSUGI TORU
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DocumentTitleAlternate 角質細胞の観察方法
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Snippet PROBLEM TO BE SOLVED: To provide a useful observation method of cuticle cells, in evaluating or screening cosmetics, skin external preparations, or the like...
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SubjectTerms BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
HUMAN NECESSITIES
HYGIENE
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PHYSICS
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS
SPECIFIC USE OF COSMETICS OR SIMILAR TOILETPREPARATIONS
SPIRITS
TESTING
VINEGAR
WINE
Title OBSERVATION METHOD OF CUTICLE CELL
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