SELF-ASSEMBLING MULTICELLULAR BODIES, AND METHOD FOR FABRICATING THREE-DIMENSIONAL BIOLOGICAL STRUCTURE USING MULTICELLULAR BODIES

• Main Authors: CYRILLE NOROTTE, GABOR FORGACS, FRANCOISE SUZANNE MARGA
• Format: Patent
• Language: English; Japanese
• Published: 04.11.2016
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide means for making it possible to assemble a three-dimensional neovascularized organ.SOLUTION: A structure and a method for tissue engineering includes a multicellular bodies containing a plurality of biological cells. Artificial tissue can be formed by arraying the plurality of multicellular bodies in a certain pattern and fusing the bodies. The arrayed body can include a filler body including a biocompatible substance which can prevent migration and ingrowth of cells from the multicellular bodies, and can prevent adhesion of cells to the multicellular bodies. Preferably, a three-dimensional construct can be assembled by printing the multicellular bodies and the filler body or by laminating them by other methods such that adjacent multicellular bodies directly contact along a contact zone having a sufficient length. Efficient and secure fusion is promoted by allowing the multicellular bodies are allowed to directly contact each other in this manner. Further, efficient and secure fusion is promoted by increasing a contact area between adjacent multicellular bodies.SELECTED DRAWING: Figure 9 【課題】３次元の血管新生化臓器を組み立てることを可能にする手段を提供する。【解決手段】ティッシュエンジニアリングのための構造体と方法は、複数の生体細胞を含む多細胞体を含む。複数の多細胞体をあるパターンで配列して融合させて、人工組織を形成させることができる。配列体は、細胞が多細胞体から移動および内殖するのを妨げると共に、細胞が多細胞体に接着するのを妨げる生体適合性物質を含むフィラー体を含むことができる。好適には十分な長さのある接触区域沿いで、隣接し合う多細胞体が直接接触するように、多細胞体とフィラー体を印刷するか、または他の方法によって積み重ねることによって、３次元コンストラクトを組み立てることができる。このように多細胞体間を直接接触させることによって、効率的かつ確実な融合を促す。また、隣接し合う多細胞体間の接触面積を増大させることによっても、効率的かつ確実な融合を促す。【選択図】図９