POLYPEPTIDE DERIVED FROM COCCOIDEA

• Main Authors: KANEKO KENJI, MIYANISHI NOBUMITSU
• Format: Patent
• Language: English; Japanese
• Published: 14.04.2016
• Subjects: CHEMISTRY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS

PROBLEM TO BE SOLVED: To provide a polypeptide derived from Coccoidea [Ceroplastes ceriferus Fabricius.] having hemolytic activity and cytotoxicity.SOLUTION: The invention provides a polypeptide derived from Ceroplastes ceriferus Fabricius. containing the amino acid sequence represented by GSGSVLVRNSGGYVATFSMA, and a hemolytic composition containing the polypeptide. The invention relates to a hemolysis method comprising contacting the polypeptide or the composition to a liquid sample containing red blood cells, and to a cytotoxic composition containing the polypeptide, as well as to an antitumor composition containing the polypeptide. The method of obtaining the polypeptide comprises fractioning a sample obtained by homogenizing entire Ceroplastes ceriferus Fabricius. by a suitable chromatography to separate the fraction representing hemolytic activity.SELECTED DRAWING: Figure 3 【課題】溶血活性および細胞毒性を有するカイガラムシ［ツノロウムシ（角蝋虫）］由来のポリペプチドの提供。【解決手段】GSGSVLVRNSGGYVATFSMAで表されるアミノ酸配列を含む、ツノロウムシ由来のポリペプチド、及び該ポリペプチドを含む溶血剤組成物。赤血球を含む液体試料に、前記ポリペプチド、又は前記組成物を接触させることを含む溶血方法、及び前記ポリペプチドを含む細胞毒性組成物、並びに前記ポリペプチドを含む抗腫瘍組成物。ツノロウムシ全体をホモジナイズした試料を、適切なクロマトグラフィで分画し、溶血活性を示す画分を分離することによって、前記ポリペプチドを取得する方法。【選択図】図３