METHOD AND COMPOSITION IN PARTICLE-BASED DETECTION OF TARGET MOLECULE USING LINKING MOLECULE

• Main Authors: XU JIANPING, JULIE PERKINS, GAO HETIAN, CELINE HU
• Format: Patent
• Language: English; Japanese
• Published: 14.01.2016
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a method and a composition in particle-based detection of a target molecule using a linking molecule.SOLUTION: A method of generating a complex comprising a nucleic acid target molecule is provided which comprises: mixing in a reaction mixture a substrate comprising a plurality of capture probes bound to the substrate, a sample suspected of containing a nucleic acid target molecule, and a linking molecule. The linking molecule is a target-specific binding portion which specifically binds to an existing nucleic acid target molecule, and when the linking molecule includes a plurality of target-specific binding portions, each of the target-specific binding portions includes one or a plurality of target-specific binding portions which specifically bind to different regions of the existing nucleic acid target molecule, and one or a plurality of capture probes specific binding portions which specifically bind to at least one of a plurality of capture probes. 【課題】連結分子を用いた標的分子の粒子ベースの検出における方法および組成物の提供。【解決手段】核酸標的分子を含む複合体の製造方法であって、基質に結合した複数の捕捉プローブを含む基質と、核酸標的分子を含有すると思われる試料と、連結分子、とを反応混合物に混合することを含み、前記連結分子が、存在する核酸標的分子に特異的に結合する標的特異的結合部分であって、該連結分子が標的特異的結合部分を複数含むとき、その標的特異的結合部分のそれぞれが、存在する核酸標的分子の異なる領域に特異的に結合する、一又は複数の標的特異的結合部分と、複数の捕捉プローブの少なくとも一つに特異的に結合する一又は複数の捕捉プローブ特異的結合部分とを含む方法。【選択図】図１