METHOD FOR DETECTING CANCER CELL

• Main Author: FUKUDA MASARU
• Format: Patent
• Language: English
• Published: 16.12.2004
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a method for detecting a cancer cell by which the cancer cell in a tissue specimen and a cell specimen can accurately be detected by reducing the occurrence of false negative and false positive as possible. SOLUTION: The method comprises soaking the tissue specimen collected from a visceral organ or the like, or the cell specimen collected from a body fluid or the like in a solution containing formaldehyde, subjecting the soaked specimen to denaturation treatment for selectively denaturing an injured DNA having injure and present in a cell nucleus of the cancer cell in the specimen to a single-stranded DNA, subjecting the treated specimen to a bonding treatment for bonding the single-stranded DNA with a labeled DNA having a chain length bondable to the single-stranded DNA, and a label enabling the observation to be carried out by an elicitation treatment such as a color-developing treatment, and arranged therein, and subjecting the treated specimen to the elicitation treatment for enabling the cancer cell to be detected by observing the presence of the labeled DNA bonded to the single-stranded DNA. COPYRIGHT: (C)2005,JPO&NCIPI