NEW NUCLEIC ACID PROBE AND NEW METHOD FOR ASSAYING NUCLEIC ACID USING THE SAME

• Main Authors: YAMADA KAZUTAKA, KURANE RYUICHIRO, KAMAGATA YOICHI, TORIMURA MASAMOTO, KURATA SHINYA, YOKOMAKU TOYOICHI, KANEKAWA TAKAHIRO
• Format: Patent
• Language: English
• Published: 10.12.2002
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide both a new nucleic acid probe for simultaneously assaying target nucleic acids with a simple apparatus when one more kinds of the target nucleic acids are present in the assaying system and a new method for assaying the nucleic acids using the probe. SOLUTION: This new nucleic acid probe for assaying the nucleic acids is characterized in that a base sequence is designed so as to reduce fluorescence intensities of both a donor dye and an acceptor dye when the probe is hybridized to the target nucleic acids and the dye is labeled in the single-stranded nucleic acid probe labeled with a plurality of fluorescent dyes so as to form at least one pair of fluorescent days causing a Forster resonance energy transfer (FRET) phenomenon, i.e., the pair of the fluorescent dye (the donor dye) convertible into the donor dye and the fluorescent dye (the acceptor dye) convertible into the acceptor dye. The new method for assaying the nucleic acids uses the probe.