DNA MICROARRAY

PROBLEM TO BE SOLVED: To improve the reliability of a hybridization process to a plurality of types of DNA probes of different Tm. SOLUTION: Spots 7 containing one type of DNA probe or the plurality of types of DNA probes of the same Tm and length are arranged in a channel 3 sequentially from the sp...

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Bibliographic Details
Main Author NISHINE TSUTOMU
Format Patent
LanguageEnglish
Published 18.10.2002
Edition7
Subjects
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TESTING
VINEGAR
WINE
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Summary:PROBLEM TO BE SOLVED: To improve the reliability of a hybridization process to a plurality of types of DNA probes of different Tm. SOLUTION: Spots 7 containing one type of DNA probe or the plurality of types of DNA probes of the same Tm and length are arranged in a channel 3 sequentially from the spot 7 containing the DNA probe of high Tm from one end side of the channel 3 to the other end side. After a sample is injected in a reservoir 5 communicating to the one end side of the channel 3 to introduce a predetermined amount of the sample into the channel 3, the sample 3 is moved from the one end side of the channel 3 to the other end side by electrophoresis. At this time, the temperature of the area is controlled according to the Tm of the DNA probe of the spot 7 at which the sample is located. With the movement of the sample, the temperature is controlled in such a way as to gradually lower temperature in the channel 3 according to the Tm of the DNA probe of the spot 7 at which the sample is located.
Bibliography:Application Number: JP20010105600