METHODS OF PRODUCING BIOCONJUGATES OF E. COLI O-ANTIGEN POLYSACCHARIDES, COMPOSITIONS THEREOF, AND METHODS OF USE THEREOF

• Main Authors: Braun, Martin Edward, Kowarik, Michael Thomas, Ibarra Yon, Patricia, Abbanat, Darren Robert, Kemmler, Stefan Jochen, Gambillara Fonck, Veronica, Carranza Sandmeier, Maria Paula, Weerdenburg, Eveline Marleen, Fae, Kellen Cristhina, Geurtsen, Jeroen, Poolman, Jan Theunis, Mally, Manuela, Burghout, Pieter Jan
• Format: Patent
• Language: Croatian; English
• Published: 08.12.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Methods of producing bioconjugates of O-antigen polysaccharides covalently linked to a carrier protein using recombinant host cells are provided. The recombinant host cells used in the methods described herein encode a particular oligosaccharyl transferase enzyme depending on the O-antigen polysacch...