Artificial chromosome for retroviral production

• Main Authors: Nina Kotsopoulou, Celeste Pallant, Conrad Vink
• Format: Patent
• Language: English
• Published: 16.11.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

This invention relates to a mammalian artificial chromosome ( also known as a MAC, satellite artificial chromosome, SATAC, an artificial chromosome expression system or an ACES) which is stably replicated and segregated alongside endogenous chromosomes in a mammalian host cell for creating more efficient retroviral packing cell lines. The artificial chromosome comprises nucleic acid sequences encoding a replication defective retroviral vector particle, preferably derived from HIV-1, HIV-2, SIV, FIV, EIAV or Visna. The retroviral vector particle comprises the RNA genome of the retroviral vector particle and genes encoding the gag (group specific antigen or HXB2 790-2292), pol (DNA polymerase) and env (viral envelope, GP160, GP120, GP41 or envelope glycoprotein) proteins. Further embodiments of the invention provide mammalian cells comprising such artificial chromosomes and methods for their use in producing retroviral particles.