PROCEDE DE PREPARATION D'UN INTERFERON LEUCOCYTAIRE HUMAIN ALPHA-2

• Main Author: V. G. DEBABOV, J. D. TSYGANKOV, A. J. CHISTOSERDOV, E. D. SVERDLOV, L. S. IZOTOVA, S. V. KOSTROV, V. E. STERKIN, V. P. KUZNETSOV, S. V. BELYAEV, G. S. MONASTYRSKAYA, I. S. SALOMATINA, G. M. DOLGANOV, S. G. ARSENIAN, S. A. TSAREV, J. I. KOZLOV, A. Y. STRONGIN, V. I. OGARKOV ET J. A. OVCHINNIKOV
• Format: Patent
• Language: French
• Published: 17.01.1986
• Edition: 4
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

L'INVENTION CONCERNE L'INDUSTRIE MICROBIOLOGIQUE.UN PROCEDE D'OBTENTION D'UN INTERFERON LEUCOCYTAIRE HUMAIN ALPHA-2 COMPREND UNE CULTURE IMMERGEE D'UNE SOUCHE PRODUCTRICE CONTENANT UN PLASMIDE AVEC UN GENE INCOPORE D'INTERFERON LEUCOCYTAIRE HUMAIN ALPHA-2, SUR UN MILIEU NUTRITIF CONTENANT DES SOURCES ASSIMILABLES DE CARBONE ET D'AZOTE, DES SELS MINERAUX ET DES AGENTS DE CROISSANCE, MILIEU SOUMIS A L'AERATION EN PRESENCE D'UN ANTIBIOTIQUE, AVEC ISOLEMENT ET PURIFICATION SUBSEQUENTS DU PRODUIT VISE.DANS LE PROCEDE, CONFORME A L'INVENTION, ON UTILISE COMME SOUCHE PRODUCTRICE LA SOUCHE PSEUDOMONAS SPECIES VG-84 PORTEUSE D'UN PLASMIDE PVG3, DEPOSEE DANS LA COLLECTION DE CULTURES DE MICRO-ORGANISMES DE L'INSTITUT PANSOVIETIQUE DE RECHERCHE SCIENTIFIQUE DES ANTIBIOTIQUES SOUS LE NUMERO 1742, ET LA CULTURE EST REALISEE EN PRESENCE DE STREPTOMYCINE OU DE TETRACYCLINE OU D'UN MELANGE DE CES ANTIBIOTIQUES. L'INTERFERON LEUCOCYTAIRE HUMAIN ALPHA-2 PRESENTE UNE ACTIVITE ANTIVIRALE ET TROUVE DES APPLICATIONS EN MEDECINE. A method for producing human leukocyte interferon alpha-2 resides in that there is carried out submerged cultivation of a producer strain Pseudomonas species VG-84 carrying a plasmid pVG3 with an inserted gene of human leukocyte interferon alpha-2, said strain being deposited in the collection of microorganism cultures at the USSR Antibiotics Research Institute under Reg. No. 1742; said strain being cultivated in a nutrient medium, containing the sources of carbon and nitrogen, mineral salts and growth stimulants, under aeration in the presence of antibiotics, i.e., streptomycin or tetracycline, or a mixture of both, followed by isolation and purification of the end product.