New enzymes for splitting lignin

• Main Authors: KIRK, THOMAS KENT, FARRELL, ROBERTA L, TIEN, MING
• Format: Patent
• Language: English; Finnish
• Published: 15.04.1993
• Edition: 5
• Subjects: APPARATUS THEREFOR; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PAPER-MAKING; PRODUCTION OF CELLULOSE; PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCESFROM CELLULOSE-CONTAINING MATERIALS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REGENERATION OF PULPING LIQUORS; SPIRITS; TEXTILES; VINEGAR; WINE

Novel lignin-degrading enzymes designated rLDM TM 1, rLDM TM 2, rLDM TM 3, rLDM TM 4, rLDM TM 5, and rLDM TM 6 are isolated and purified to the essentially pure form, wherein each rLDM TM is substantially free of other rLDM TM and native proteins, from the extracellular medium of a novel mutant microbe. The novel mutant, designated SC26, produces large amounts of the rLDM TM , thus facilitating the isolation and purification of them. These rLDM TM are useful in pulping processes to degrade and/or modify lignin.