Modificación genómica usando sistemas de polinucleótido guía/endonucleasa Cas y métodos de uso

• Main Authors: YOUNG, Joshua K, PATTEN, Phillip A, CIGAN, Andrew
• Format: Patent
• Language: Spanish
• Published: 05.11.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Un polinucleótido guía que comprende: (i) un primer dominio de secuencia de nucleótidos que es complementario a una secuencia de nucleótidos en un ADN diana; y, (ii) un segundo dominio de secuencia de nucleótidos que interactúa con una endonucleasa Cas, en donde el primer dominio de secuencia de nucleótidos y el segundo dominio de secuencia de nucleótidos están compuestos por ácidos desoxirribonucleicos (ADN), ácidos ribonucleicos (ARN) o una combinación de los mismos, en donde el polinucleótido guía comprende ADN. Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell.