Producción de ácidos grasos poliinsaturados en organismos heterólogos usando sistemas de policétido sintasa de AGPI

• Main Authors: METZ, JAMES G, KUNER, JERRY M, LIPPMEIER, JAMES CASEY
• Format: Patent
• Language: Spanish
• Published: 02.02.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE

Molécula de ácido nucleico aislada que comprende una secuencia de ácido nucleico que codifica para una acil- CoA sintetasa (ACoAS) que cataliza la conversión de ácidos grasos libres (AGL) de AGPI de cadena larga en acil- CoA, en la que la secuencia de ácido nucleico codifica para una acil-CoA sintetasa (ACoAS) que es idéntica en al menos el 75% a una ACoAS que tiene una secuencia de aminoácidos de SEQ ID NO: 83. Disclosed are plants that have been genetically modified to express a PKS-like system for the production of PUFAs (a PUFA PKS system), wherein oils produced by the plant contain at least one PUFA produced by the PUFA PKS system and are free of the mixed shorter-chain and less unsaturated PUFAs that are fatty acid products produced by the modification of products of the FAS system in the standard fatty acid pathways. Also disclosed are the oil seeds, oils, and products comprising such oils produced by this system, as well as methods for producing such plant.