Proceso para el cultivo de microorganismos de la especie Thraustochytrieales

• Main Authors: LUY, MARKUS, RUESING, MATTHIAS
• Format: Patent
• Language: Spanish
• Published: 20.09.2012
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Procedimiento para el cultivo de microorganismos de la especie Thraustochytriales, caracterizado porque losmicroorganismos se cultivan en un medio de fermentación que presenta CaCO3 como medio esencial para laestabilización del valor de pH, que se presenta con un contenido de 3-15 g/l en el medio de fermentación, siendoentonces CaCO3 un medio esencial para la estabilización del valor de pH si la suma de diferencias de valores de pH,que se pueden medir con o sin adición de ácidos, - en cada caso con la adición de CaCO3 según la invención - esmenor o igual a 1. The invention relates to an optimized method for the production of PUFAs by cultivating microorganisms belonging to the group of Stramenopiles in a fermentation medium that is pH-stabilized using calcium carbonate and comprises 3-15 g/L CaCO3, whereupon the PUFAs are isolated from the microorganisms and/or the medium. The invention particularly relates to novel optimized media having a different CaCO3 content. By using adequate quantities of CaCO3, the process can be significantly simplified during fermentation while greater quantities of DHA can be obtained at an increased oil content in the biomass. They allow microorganisms belonging to the Stramenopiles to be fermented without controlling the pH, thereby substantially improving and significantly simplifying PUFA production.