IMPROVED PADLOCK PROBES FOR NUCLEIC ACID DETECTION BY HYPERBRANCHED ROLLING CIRCLE AMPLIFICATION

• Main Authors: CORTÉS LEDESMA, Felipe, GARCÍA EXPÓSITO, Laura, LÓPEZ DE ALBA, Ernesto, MUÑOZ BARRERA, Marta, GÓMEZ MARÍN, Carlos
• Format: Patent
• Language: English; French; German
• Published: 25.09.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The present invention relates to a HRCA method comprising the steps of a) providing a nucleic acid structure formed by the hybridization of at least one padlock with a target nucleic acid, b) circularising the hybridised padlock, c) subjecting the ligated circularized padlock probe to rolling circle...