TRANSPOSITION INTO NATIVE CHROMATIN FOR PERSONAL EPIGENOMICS
Provided herein is a method for analyzing polynucleotides such as genomic DNA. In certain embodiments, the method comprises: (a) treating chromatin isolated from a population of cells with an insertional enzyme complex to produce tagged fragments of genomic DNA; (b) sequencing a portion of the tagge...
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| Main Authors | , , , |
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| Format | Patent |
| Language | English French German |
| Published |
14.02.2024
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| Subjects | |
| Online Access | Get full text |
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| Abstract | Provided herein is a method for analyzing polynucleotides such as genomic DNA. In certain embodiments, the method comprises: (a) treating chromatin isolated from a population of cells with an insertional enzyme complex to produce tagged fragments of genomic DNA; (b) sequencing a portion of the tagged fragments to produce a plurality of sequence reads; and (c) making an epigenetic map of a region of the genome of the cells by mapping information obtained from the sequence reads to the region. A kit for performing the method is also provided. |
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| AbstractList | Provided herein is a method for analyzing polynucleotides such as genomic DNA. In certain embodiments, the method comprises: (a) treating chromatin isolated from a population of cells with an insertional enzyme complex to produce tagged fragments of genomic DNA; (b) sequencing a portion of the tagged fragments to produce a plurality of sequence reads; and (c) making an epigenetic map of a region of the genome of the cells by mapping information obtained from the sequence reads to the region. A kit for performing the method is also provided. |
| Author | BUENROSTRO, Jason D CHANG, Howard Y GREENLEAF, William J GIRESI, Paul |
| Author_xml | – fullname: GREENLEAF, William J – fullname: CHANG, Howard Y – fullname: GIRESI, Paul – fullname: BUENROSTRO, Jason D |
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| DocumentTitleAlternate | TRANSPOSITION EN CHROMATINE NATIVE POUR L'ÉPIGÉNOMIQUE PERSONNELLE TRANSPOSITION IN NATIVES CHROMATIN FÜR PERSÖNLICHE EPIGENOMIK |
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| RelatedCompanies | The Board of Trustees of the Leland Stanford Junior University |
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| Snippet | Provided herein is a method for analyzing polynucleotides such as genomic DNA. In certain embodiments, the method comprises: (a) treating chromatin isolated... |
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| SubjectTerms | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| Title | TRANSPOSITION INTO NATIVE CHROMATIN FOR PERSONAL EPIGENOMICS |
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