ARTIFICIAL AXON BUNDLE

• Main Authors: SHINDO, Tomoko, KIMURA, Hiroo, KAWADA, Jiro, OKANO, Hideyuki, NISHIJIMA, Takayuki, SHIBATA, Shinsuke, TOITA, Sayaka
• Format: Patent
• Language: English; French; German
• Published: 17.04.2024
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES; MEDICAL OR VETERINARY SCIENCE; METALLURGY; METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to an axon bundle obtained by a method of culturing a nerve cell aggregation having one spheroid and an axon bundle extending from the spheroidwherein the nerve cell aggregation consisting of a plurality of neurons having a cell body and an axon obtained by a method comprising the following step:supplying a culture medium to one first chamber, one second chamber and one channel having the length of at least 1 mm, the width of 100 to 150 µm and the hight of 100 to 200 µm, which connecting said first chamber and second chamber, wherein said first chamber, second chamber, and channel are contained in one of the modules disposed in a culture plate,seeding the first chamber with a nerve cell derived from a stem cell or a spheroid of primary cultured nerve cells; andculturing said nerve cell, thereby growing an axon bundle from said spheroid andextending them into said channel,wherein the length of the axon bundle obtained is 1 mm or more, and the diameter of the axon bundles is 50 µm or more.