METHODS TO MINIMIZE PHOTODAMAGE DURING NUCLEIC ACID AND PEPTIDE SEQUENCING
Provided herein are methods and integrated devices for improved sequencing of nucleic acid and peptide biomolecules. The present disclosure relates to improved mechanisms for protecting a luminescent label from photo-induced damage through the use of quenching moieties. Further provided herein are m...
Saved in:
Main Authors | , , |
---|---|
Format | Patent |
Language | English French German |
Published |
28.08.2024
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Provided herein are methods and integrated devices for improved sequencing of nucleic acid and peptide biomolecules. The present disclosure relates to improved mechanisms for protecting a luminescent label from photo-induced damage through the use of quenching moieties. Further provided herein are methods for improved immobilization of quenching moieties and other molecules of interest through functionalization with chemical moieties, such as click chemistry handles, capable of participating in cross-linking reactions. Quenching moieties may be immobilized to the surface of a sample well in a sequencing substrate or apparatus in a manner that minimizes or eliminates photobleaching of the labeled molecule. The disclosed methods provide for minimized photodamage, increased sensitivity, accuracy and length of reads during nucleic acid and polypeptide sequencing. |
---|---|
AbstractList | Provided herein are methods and integrated devices for improved sequencing of nucleic acid and peptide biomolecules. The present disclosure relates to improved mechanisms for protecting a luminescent label from photo-induced damage through the use of quenching moieties. Further provided herein are methods for improved immobilization of quenching moieties and other molecules of interest through functionalization with chemical moieties, such as click chemistry handles, capable of participating in cross-linking reactions. Quenching moieties may be immobilized to the surface of a sample well in a sequencing substrate or apparatus in a manner that minimizes or eliminates photobleaching of the labeled molecule. The disclosed methods provide for minimized photodamage, increased sensitivity, accuracy and length of reads during nucleic acid and polypeptide sequencing. |
Author | LI, An CHEN, Guojun THURSTON, Thomas, Raymond |
Author_xml | – fullname: THURSTON, Thomas, Raymond – fullname: CHEN, Guojun – fullname: LI, An |
BookMark | eNqNyjsKwkAQANAttPB3h7mAILqC7bIzJiPux2S3sQlBxko2gXh_tPAAVq95SzUrQ5GFujhKdcAWUgDHnh3fCWIdUkDjTEWAuWFfgc_2SmzBWEYwHiFSTIwELd0yefs9azV_9q9JNj9XCs6UbL2VcehkGvuHFHl3FPV-p48nbfThj_IBHW8uog |
ContentType | Patent |
DBID | EVB |
DatabaseName | esp@cenet |
DatabaseTitleList | |
Database_xml | – sequence: 1 dbid: EVB name: esp@cenet url: http://worldwide.espacenet.com/singleLineSearch?locale=en_EP sourceTypes: Open Access Repository |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Medicine Chemistry Sciences Physics |
DocumentTitleAlternate | VERFAHREN ZUR MINIMIERUNG VON LICHTSCHÄDEN WÄHREND DER NUKLEINSÄURE- UND PEPTIDSEQUENZIERUNG PROCÉDÉS PERMETTANT DE RÉDUIRE AU MINIMUM LA PHOTODÉGRADATION PENDANT LE SÉQUENÇAGE D'ACIDE NUCLÉIQUE ET DE PEPTIDE |
ExternalDocumentID | EP4204584A4 |
GroupedDBID | EVB |
ID | FETCH-epo_espacenet_EP4204584A43 |
IEDL.DBID | EVB |
IngestDate | Fri Sep 06 06:15:19 EDT 2024 |
IsOpenAccess | true |
IsPeerReviewed | false |
IsScholarly | false |
Language | English French German |
LinkModel | DirectLink |
MergedId | FETCHMERGED-epo_espacenet_EP4204584A43 |
Notes | Application Number: EP20210870429 |
OpenAccessLink | https://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240828&DB=EPODOC&CC=EP&NR=4204584A4 |
ParticipantIDs | epo_espacenet_EP4204584A4 |
PublicationCentury | 2000 |
PublicationDate | 20240828 |
PublicationDateYYYYMMDD | 2024-08-28 |
PublicationDate_xml | – month: 08 year: 2024 text: 20240828 day: 28 |
PublicationDecade | 2020 |
PublicationYear | 2024 |
RelatedCompanies | Quantum-Si Incorporated |
RelatedCompanies_xml | – name: Quantum-Si Incorporated |
Score | 3.5675154 |
Snippet | Provided herein are methods and integrated devices for improved sequencing of nucleic acid and peptide biomolecules. The present disclosure relates to improved... |
SourceID | epo |
SourceType | Open Access Repository |
SubjectTerms | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE |
Title | METHODS TO MINIMIZE PHOTODAMAGE DURING NUCLEIC ACID AND PEPTIDE SEQUENCING |
URI | https://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240828&DB=EPODOC&locale=&CC=EP&NR=4204584A4 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3dT8IwEL8gfr4pasSv9MHsbRHGGPBADLTFjbgPZTPGFzK6LuFlEJnx3_faAPqib821adprf3ft9e4KcNdpCicXWcNsiF7LtNMcIZX2MlOKHLcPbmjZVsHJfuC4iT1-a79VYL6JhdF5Qr90ckRElEC8l1peL3-MWEz7Vq7uZ3MkLR5GcZ8Z69uxytdldQ027PMoZCE1KMWSEbz0bUu_CA7sHdhVp2iVZp-_DlVQyvK3Rhkdw16EnRXlCVRkUYNDuvl4rQYH_vq9uwb72kFTrJC4BuHqFMY-j92QTUgcEt8LUPq8cxK5YRwylKCPnLBEuTiQIKFP3KNkQD1GBgEjEY9ij3Ey4c8JDyi2OQMy4jF1TRzedMuKKY-2E2mdQ7VYFPICSCvrSGl3MqubCztz2qm0Ukc0LVwDhGivUYf6n91c_lN3BUeKp8qEanWvoVp-fMob1MHl7FZz7xsDbYTe |
link.rule.ids | 230,309,786,891,25594,76903 |
linkProvider | European Patent Office |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3NT8IwFH9B_MCbokb87MHstohjbHAgBtrihuxDGYZ4IaPrEi6DyIz_vm8NoBe9NW3TtK_9vZf3WYA7-0FYqUjqel20G7oZpwipuJ3oUqT4fPBBy2aRnOz5ljM2B5PmpATzTS6MqhP6pYojIqIE4j1X_Hr5Y8RiKrZydT-bY9fisR91mLbWjot6XUZLY70ODwMWUI1SbGn-a8c0lEewa-7Aro0aodKU3npFUsryt0TpH8FeiItl-TGUZFaFCt18vFaFA2_t767CvgrQFCvsXINwdQIDj0dOwEYkCojn-sh93jkJnSAKGHLQJ07YuAhxIP6YDrlLSZe6jHR9RkIeRi7jZMRfxtynOOcUSJ9H1NFxe9MtKaY83B6kcQblbJHJcyCNxJbStBOjlQozsZqxNGJLPBh4BwjRdr0GtT-Xufhn7BYqTuQNp0PXf76Ew4K-hTnVaF1BOf_4lNcoj_PZjaLkN16Th8g |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Apatent&rft.title=METHODS+TO+MINIMIZE+PHOTODAMAGE+DURING+NUCLEIC+ACID+AND+PEPTIDE+SEQUENCING&rft.inventor=THURSTON%2C+Thomas%2C+Raymond&rft.inventor=CHEN%2C+Guojun&rft.inventor=LI%2C+An&rft.date=2024-08-28&rft.externalDBID=A4&rft.externalDocID=EP4204584A4 |