RAPID SINGLE-STEP GRADIENT METHOD FOR THE GENERATION OF NANOANTIBODIES

• Main Authors: LÓPEZ, Natalia, SALINAS, Constanza, PINTO, Teresa, CHEUQUEMILLA, Yorka, UBERTI, Benjamín, JARA, Ronald, MULLER, Ananda, VALENZUELA, Guillermo, HIMELREICHS, Johanna, CUEVAS, Alexei, ROJAS, Alejandro, MIRANDA, Zaray
• Format: Patent
• Language: English; French; German
• Published: 07.08.2024
• Subjects: CHEMISTRY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; TESTING

One-step rapid gradient method for rapid generation of nanoantibodies (HHV) against any antigen. Where the method comprises the separation of nanoantibodies against a specific antigen from a nanoantibody expression library through the following steps:(a) binding the antigen of interest to beads made of protein-binding polymers(b) incubating the microorganisms of the expression library with the pearls of stage (a),(c) placing the beads of step (b) in a tube with an inert medium with a density greater than or equal to 1 g/mL and centrifuge for 45 s to 2 minutes at a rate between 150 and 250 g;d) discarding the upper fraction and supernatant with the free microorganisms, and obtain the beads with the linked microorganisms, which correspond to those that express HHV that recognizes the antigen.