RAPID SINGLE-STEP GRADIENT METHOD FOR THE GENERATION OF NANOANTIBODIES

One-step rapid gradient method for rapid generation of nanoantibodies (HHV) against any antigen. Where the method comprises the separation of nanoantibodies against a specific antigen from a nanoantibody expression library through the following steps:(a) binding the antigen of interest to beads made...

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Main Authors LÓPEZ, Natalia, SALINAS, Constanza, PINTO, Teresa, CHEUQUEMILLA, Yorka, UBERTI, Benjamín, JARA, Ronald, MULLER, Ananda, VALENZUELA, Guillermo, HIMELREICHS, Johanna, CUEVAS, Alexei, ROJAS, Alejandro, MIRANDA, Zaray
Format Patent
LanguageEnglish
French
German
Published 22.03.2023
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Abstract One-step rapid gradient method for rapid generation of nanoantibodies (HHV) against any antigen. Where the method comprises the separation of nanoantibodies against a specific antigen from a nanoantibody expression library through the following steps:(a) binding the antigen of interest to beads made of protein-binding polymers(b) incubating the microorganisms of the expression library with the pearls of stage (a),(c) placing the beads of step (b) in a tube with an inert medium with a density greater than or equal to 1 g/mL and centrifuge for 45 s to 2 minutes at a rate between 150 and 250 g;d) discarding the upper fraction and supernatant with the free microorganisms, and obtain the beads with the linked microorganisms, which correspond to those that express HHV that recognizes the antigen.
AbstractList One-step rapid gradient method for rapid generation of nanoantibodies (HHV) against any antigen. Where the method comprises the separation of nanoantibodies against a specific antigen from a nanoantibody expression library through the following steps:(a) binding the antigen of interest to beads made of protein-binding polymers(b) incubating the microorganisms of the expression library with the pearls of stage (a),(c) placing the beads of step (b) in a tube with an inert medium with a density greater than or equal to 1 g/mL and centrifuge for 45 s to 2 minutes at a rate between 150 and 250 g;d) discarding the upper fraction and supernatant with the free microorganisms, and obtain the beads with the linked microorganisms, which correspond to those that express HHV that recognizes the antigen.
Author PINTO, Teresa
HIMELREICHS, Johanna
MULLER, Ananda
ROJAS, Alejandro
CHEUQUEMILLA, Yorka
UBERTI, Benjamín
LÓPEZ, Natalia
VALENZUELA, Guillermo
JARA, Ronald
CUEVAS, Alexei
MIRANDA, Zaray
SALINAS, Constanza
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– fullname: SALINAS, Constanza
– fullname: PINTO, Teresa
– fullname: CHEUQUEMILLA, Yorka
– fullname: UBERTI, Benjamín
– fullname: JARA, Ronald
– fullname: MULLER, Ananda
– fullname: VALENZUELA, Guillermo
– fullname: HIMELREICHS, Johanna
– fullname: CUEVAS, Alexei
– fullname: ROJAS, Alejandro
– fullname: MIRANDA, Zaray
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DocumentTitleAlternate SCHNELLES EINSTUFIGES GRADIENTENVERFAHREN ZUR ERZEUGUNG VON NANOANTIKÖRPERN
MÉTHODE DE GRADIENT RAPIDE À UN SEUL PASSAGE POUR LA PRODUCTION D'ANTICORPS
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Snippet One-step rapid gradient method for rapid generation of nanoantibodies (HHV) against any antigen. Where the method comprises the separation of nanoantibodies...
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SubjectTerms BEER
BIOCHEMISTRY
CHEMISTRY
COMBINATORIAL CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES
MEASURING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TESTING
VINEGAR
WINE
Title RAPID SINGLE-STEP GRADIENT METHOD FOR THE GENERATION OF NANOANTIBODIES
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