MICROFLUIDIC DEVICES AND METHODS FOR CELL ANALYSIS
In a method for analyzing cells, a sample fluid comprising a suspending medium and cells is fed to a microfluidic device comprising at least one cell processing unit (10) comprising a trapping region (12), a reaction unit (20), and an outlet arrangement (30). The trapping region is delimited by at l...
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Format | Patent |
Language | English French German |
Published |
04.01.2023
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Abstract | In a method for analyzing cells, a sample fluid comprising a suspending medium and cells is fed to a microfluidic device comprising at least one cell processing unit (10) comprising a trapping region (12), a reaction unit (20), and an outlet arrangement (30). The trapping region is delimited by at least an input valve (13) and a sieve valve (14), in particular a v-type valve that is capable of retaining the cells while letting fluids pass. The method comprises trapping cells in the trapping region, subsequently establishing a flow of a reaction fluid through the trapping region while the sieve valve (14) assumes said open state, such that the reaction fluid transfers the trapped cells from the trapping region (12) into the reaction unit (20), decomposing the transferred cells into cell fragments through a decomposition process, collecting the cell fragments in the outlet arrangement, and analyzing the cell fragments. |
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AbstractList | In a method for analyzing cells, a sample fluid comprising a suspending medium and cells is fed to a microfluidic device comprising at least one cell processing unit (10) comprising a trapping region (12), a reaction unit (20), and an outlet arrangement (30). The trapping region is delimited by at least an input valve (13) and a sieve valve (14), in particular a v-type valve that is capable of retaining the cells while letting fluids pass. The method comprises trapping cells in the trapping region, subsequently establishing a flow of a reaction fluid through the trapping region while the sieve valve (14) assumes said open state, such that the reaction fluid transfers the trapped cells from the trapping region (12) into the reaction unit (20), decomposing the transferred cells into cell fragments through a decomposition process, collecting the cell fragments in the outlet arrangement, and analyzing the cell fragments. |
Author | Stavrakis, Stavros deMello, Andrew James Aebersold, Rudolf Xue, Peng Cao, Xiaobao |
Author_xml | – fullname: deMello, Andrew James – fullname: Xue, Peng – fullname: Stavrakis, Stavros – fullname: Aebersold, Rudolf – fullname: Cao, Xiaobao |
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DocumentTitleAlternate | DISPOSITIFS MICROFLUIDIQUES ET PROCÉDÉS D'ANALYSE DE CELLULES MIKROFLUIDISCHE VORRICHTUNGEN UND VERFAHREN ZUR ZELLANALYSE |
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Snippet | In a method for analyzing cells, a sample fluid comprising a suspending medium and cells is fed to a microfluidic device comprising at least one cell... |
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SubjectTerms | CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PHYSICS TESTING TRANSPORTING |
Title | MICROFLUIDIC DEVICES AND METHODS FOR CELL ANALYSIS |
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