METHODS AND SYSTEMS FOR OPTIMIZING BUFFER CONDITIONS WITH LIQUID CHROMATOGRAPHY

A plurality of chromatograms is run using a salt gradient with a range of constant pH values or using a pH gradient with a range of constant salt concentrations. A chromatography optimization algorithm can be used to identify at least one salt gradient chromatogram or at least one pH gradient chroma...

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Bibliographic Details
Main Authors BAEK, Jung Un, LIU, Xiaodong, TRACY, Mark Lee, POHL, Christopher A, LIEHR, Shanhua Lin
Format Patent
LanguageEnglish
French
German
Published 16.12.2020
Subjects
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Summary:A plurality of chromatograms is run using a salt gradient with a range of constant pH values or using a pH gradient with a range of constant salt concentrations. A chromatography optimization algorithm can be used to identify at least one salt gradient chromatogram or at least one pH gradient chromatogram to establish optimized buffer conditions. The chromatography optimization algorithm can include a total number of peaks algorithm, a peak-to-valley algorithm, and/or a peak capacity algorithm.
Bibliography:Application Number: EP20200178908