METHOD OF PURIFYING POLYPEPTIDES

The present invention relates to improved processes for purifying polypeptides of interest by increasing the amount of a polypeptide of interest bound to an anion-exchange matrix relative to the amount of one or more impurities bound to the anion-exchange matrix. This effect is achieved by adding a...

Full description

Saved in:
Bibliographic Details
Main Authors Smrdelj, Paul, Schroeder, Magnus, Charlton, Adam, Walker, Ian, Napoli, Mark, Stowers, Anthony, Pirzas, Vicky
Format Patent
LanguageEnglish
French
German
Published 30.09.2020
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
Online AccessGet full text

Cover

Abstract The present invention relates to improved processes for purifying polypeptides of interest by increasing the amount of a polypeptide of interest bound to an anion-exchange matrix relative to the amount of one or more impurities bound to the anion-exchange matrix. This effect is achieved by adding a chemical compound in the process, which by also binding to the anion-exchange matrix due to a change that is opposite to the change of the anion-exchange matrix, reduces the binding of impurities more than the binding of the polypeptide of interest.
AbstractList The present invention relates to improved processes for purifying polypeptides of interest by increasing the amount of a polypeptide of interest bound to an anion-exchange matrix relative to the amount of one or more impurities bound to the anion-exchange matrix. This effect is achieved by adding a chemical compound in the process, which by also binding to the anion-exchange matrix due to a change that is opposite to the change of the anion-exchange matrix, reduces the binding of impurities more than the binding of the polypeptide of interest.
Author Charlton, Adam
Pirzas, Vicky
Stowers, Anthony
Smrdelj, Paul
Schroeder, Magnus
Walker, Ian
Napoli, Mark
Author_xml – fullname: Smrdelj, Paul
– fullname: Schroeder, Magnus
– fullname: Charlton, Adam
– fullname: Walker, Ian
– fullname: Napoli, Mark
– fullname: Stowers, Anthony
– fullname: Pirzas, Vicky
BookMark eNrjYmDJy89L5WRQ8HUN8fB3UfB3UwgIDfJ0i_T0c1cI8PeJDHANCPF0cQ3mYWBNS8wpTuWF0twMCm6uIc4euqkF-fGpxQWJyal5qSXxrgHG5oamxqZmjobGRCgBAKGgI34
ContentType Patent
DBID EVB
DatabaseName esp@cenet
DatabaseTitleList
Database_xml – sequence: 1
  dbid: EVB
  name: esp@cenet
  url: http://worldwide.espacenet.com/singleLineSearch?locale=en_EP
  sourceTypes: Open Access Repository
DeliveryMethod fulltext_linktorsrc
Discipline Medicine
Chemistry
Sciences
DocumentTitleAlternate PROCÉDÉ DE PURIFICATION DE POLYPEPTIDES
VERFAHREN ZUR AUFREINIGUNG VON POLYPEPTIDEN
ExternalDocumentID EP3715356A1
GroupedDBID EVB
ID FETCH-epo_espacenet_EP3715356A13
IEDL.DBID EVB
IngestDate Fri Aug 23 07:05:12 EDT 2024
IsOpenAccess true
IsPeerReviewed false
IsScholarly false
Language English
French
German
LinkModel DirectLink
MergedId FETCHMERGED-epo_espacenet_EP3715356A13
Notes Application Number: EP20200162007
OpenAccessLink https://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200930&DB=EPODOC&CC=EP&NR=3715356A1
ParticipantIDs epo_espacenet_EP3715356A1
PublicationCentury 2000
PublicationDate 20200930
PublicationDateYYYYMMDD 2020-09-30
PublicationDate_xml – month: 09
  year: 2020
  text: 20200930
  day: 30
PublicationDecade 2020
PublicationYear 2020
RelatedCompanies CSL Limited
RelatedCompanies_xml – name: CSL Limited
Score 3.2912188
Snippet The present invention relates to improved processes for purifying polypeptides of interest by increasing the amount of a polypeptide of interest bound to an...
SourceID epo
SourceType Open Access Repository
SubjectTerms BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
Title METHOD OF PURIFYING POLYPEPTIDES
URI https://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200930&DB=EPODOC&locale=&CC=EP&NR=3715356A1
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3NS8MwFH-MKepNp-L8IgfprViWLnOHIq5JbcW2YXaynUbTprDLHK7iv-9r7aYXvYUEXpIHvzze1y8ANzTV6ElXLcpsmJu2zpmZUlaY-q6w0qIY5HmdPQ8j5k_sp2l_2oLFphem5gn9rMkREVEZ4r2s3-vVTxCL17WV61u1wKm3ey9xuNF4x1Won1oGHzlCxjx2DdfFkRGNHTpAaPfZAzpKOxXrVkWzL15HVVPK6rdF8Q5hV6KwZXkELb3swL67-XitA3thk-_GYQO99TGQUCR-zEnsETkZB94siB6JjJ9nUsgk4OLlBIgnEtc3cav59lpzIbeHoqfQRm9fnwFJNVr8rMgQDcoe0lwx1SsY66e9XFFmqS50_xRz_s_aBRxU-vkudbiEdvn-oa_QnpbqutbEF1DAduI
link.rule.ids 230,308,780,885,25564,76547
linkProvider European Patent Office
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1LT8MwDLamgRg3GCDGswfUW0W1tCk7VIj1QQt9RKND26lq2lTaZUysiL-PW7rBBW5WIjmJpS-O7dgGuCGZQEu6TlGmo0LRREGVjNBSEXelmpWlURRN9DyMqDfVnmb6rAOLTS5MUyf0symOiIjKEe9Vc1-vfpxYdvO3cn3LFzj0du8mpi231nHt6ieqbI9Nh8V2bMmWhZQcTUxiILR1-oCG0o5RN9ytX06v4zopZfVbo7gHsMuQ2bI6hI5Y9qFnbRqv9WEvbOPdSLbQWx-BFDqJF9tS7EpsOvHduR89SiwO5sxhiW87L8cguU5ieQoulW6PlTpsuylyAl209sUpSJlAjZ-XOaKBayNScMqHJaV6Niw4oSofwOBPNmf_zF1Dz0vCIA386Pkc9mtZfX97uIBu9f4hLlG3VvyqkcoXDFh50w
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Apatent&rft.title=METHOD+OF+PURIFYING+POLYPEPTIDES&rft.inventor=Smrdelj%2C+Paul&rft.inventor=Schroeder%2C+Magnus&rft.inventor=Charlton%2C+Adam&rft.inventor=Walker%2C+Ian&rft.inventor=Napoli%2C+Mark&rft.inventor=Stowers%2C+Anthony&rft.inventor=Pirzas%2C+Vicky&rft.date=2020-09-30&rft.externalDBID=A1&rft.externalDocID=EP3715356A1