METHOD OF PURIFYING POLYPEPTIDES

The present invention relates to improved processes for purifying polypeptides of interest by increasing the amount of a polypeptide of interest bound to an anion-exchange matrix relative to the amount of one or more impurities bound to the anion-exchange matrix. This effect is achieved by adding a...

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Bibliographic Details
Main Authors Smrdelj, Paul, Schroeder, Magnus, Charlton, Adam, Walker, Ian, Napoli, Mark, Stowers, Anthony, Pirzas, Vicky
Format Patent
LanguageEnglish
French
German
Published 30.09.2020
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The present invention relates to improved processes for purifying polypeptides of interest by increasing the amount of a polypeptide of interest bound to an anion-exchange matrix relative to the amount of one or more impurities bound to the anion-exchange matrix. This effect is achieved by adding a chemical compound in the process, which by also binding to the anion-exchange matrix due to a change that is opposite to the change of the anion-exchange matrix, reduces the binding of impurities more than the binding of the polypeptide of interest.
Bibliography:Application Number: EP20200162007