METHOD FOR DETECTING LIPID BILAYER MEMBRANE PARTICLES OR FRAGMENTS THEREOF

• Main Authors: TAKAO, Masashi, NAGAI, Yutaka, ITO, Masami
• Format: Patent
• Language: English; French; German
• Published: 14.07.2021
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

Provided is a means by which a signal-to-noise ratio (S/N ratio) can be improved at the time of detection of lipid bilayer membrane particles (lipid vesicles) in which the amount of a surface antigen is small since the size of the particles is small and/or the amount of the particles existing in a sample is also small. The present application provides, as a means for solving the above-described problem, a detection technique that relates to a method for detecting lipid bilayer membrane particles or fragments thereof having predetermined molecules existing on surfaces in a biological sample collected from a subject, the method including: adding a dye staining a lipid bilayer membrane to the biological sample; adding a substance specifically binding to the predetermined molecules to the biological sample; trapping the substance to separate the lipid bilayer membrane particles or fragments thereof having predetermined molecules existing on surfaces; and detecting the separated lipid bilayer membrane particles or fragments thereof on the basis of emission of the dye.