CRISPR-BASED COMPOSITIONS AND METHODS OF USE

• Main Authors: COLLINGWOOD, Michael, Allen, BEHLKE, Mark, Aaron, RETTIG, Garrett, Richard, JACOBI, Ashley, Mae, SCHUBERT, Mollie, Sue
• Format: Patent
• Language: English; French; German
• Published: 25.10.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

This invention pertains to modified compositions for use in CRISPR systems, and their methods of use. In particular, length-modified and chemically-modified forms of crRNA and tracrRNA are described for use as a reconstituted guide RNA for interaction with Cas9 of CRIPSR systems. The resultant lengt...