CRISPR-BASED COMPOSITIONS AND METHODS OF USE

This invention pertains to modified compositions for use in CRISPR systems, and their methods of use. In particular, length-modified and chemically-modified forms of crRNA and tracrRNA are described for use as a reconstituted guide RNA for interaction with Cas9 of CRIPSR systems. The resultant lengt...

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Main Authors COLLINGWOOD, Michael, Allen, BEHLKE, Mark, Aaron, RETTIG, Garrett, Richard, JACOBI, Ashley, Mae, SCHUBERT, Mollie, Sue
Format Patent
LanguageEnglish
French
German
Published 25.10.2017
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Abstract This invention pertains to modified compositions for use in CRISPR systems, and their methods of use. In particular, length-modified and chemically-modified forms of crRNA and tracrRNA are described for use as a reconstituted guide RNA for interaction with Cas9 of CRIPSR systems. The resultant length-modified and chemically-modified forms of crRNA and tracrRNA are economical to produce and can be tailored to have unique properties relevant to their biochemical and biological activity in the context of the CRIPSR Cas9 endonuclease system.
AbstractList This invention pertains to modified compositions for use in CRISPR systems, and their methods of use. In particular, length-modified and chemically-modified forms of crRNA and tracrRNA are described for use as a reconstituted guide RNA for interaction with Cas9 of CRIPSR systems. The resultant length-modified and chemically-modified forms of crRNA and tracrRNA are economical to produce and can be tailored to have unique properties relevant to their biochemical and biological activity in the context of the CRIPSR Cas9 endonuclease system.
Author JACOBI, Ashley, Mae
COLLINGWOOD, Michael, Allen
BEHLKE, Mark, Aaron
RETTIG, Garrett, Richard
SCHUBERT, Mollie, Sue
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DocumentTitleAlternate COMPOSITIONS À BASE DE CRISPR ET LEURS PROCÉDÉS D'UTILISATION
ZUSAMMENSETZUNGEN AUF CRISPR-BASIS UND VERFAHREN ZUR VERWENDUNG
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Snippet This invention pertains to modified compositions for use in CRISPR systems, and their methods of use. In particular, length-modified and chemically-modified...
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SubjectTerms BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
Title CRISPR-BASED COMPOSITIONS AND METHODS OF USE
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