MODIFIED SINGLE VARIABLE DOMAIN ANTIBODIES WITH REDUCED BINDING TO ANTI-DRUG-ANTIBODIES

• Main Authors: ASHMAN, CLAIRE, HOLLAND, CLAIRE, LEWIS, ALAN PETER, DE WILDT, RUDOLF M.T, MORLEY, PETER, SANDAL, THOMAS, STEWARD, MICHAEL, BIRCHLER, MARY
• Format: Patent
• Language: English; French; German
• Published: 13.07.2016
• Subjects: CHEMISTRY; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES

The present invention relates to modified proteins and peptides that have reduced ability to bind to pre-existing antibodies. Such modified protein/peptide molecules can comprise C-terminal additions, extensions or tags and/or certain amino acid substitutions. Such modified molecules (e.g. fusions and conjugates) comprise proteins, peptides, antigen binding molecules, antibodies or antibody fragments such as single variable domains e.g. human immunoglobulin (antibody) single variable domains, and also single variable domains derived from non-human sources such as a llama or camel, e.g. a VHH including a nanobody „¢ (described in e.g. WO 94/04678 and WO 95/04079 inter alia). The invention further relates to uses, formulations, compositions comprising such modified C terminally extended and/or amino acid substituted molecules and also to methods of production and expression of these molecules.