ISOLATION AND DEGLYCOSYLATION OF GLYCOPROTEINS

• Main Authors: FULTON, SCOTT P, MURPHY, STEVEN M
• Format: Patent
• Language: English; French; German
• Published: 09.04.2014
• Subjects: CHEMISTRY; COMPOSITIONS BASED THEREON; DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS; DERIVATIVES THEREOF; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; ORGANIC MACROMOLECULAR COMPOUNDS; PHYSICS; POLYSACCHARIDES; TESTING; THEIR PREPARATION OR CHEMICAL WORKING-UP

The invention provides more rapid and cost-effective methods of deglycosylating target glycoproteins. In methods of the invention, the target glycoprotein is isolated from initial samples, which may contain multiple other glycoproteins, by subjecting the initial sample to a solid phase containing an affinity ligand, such as a deglycosylated antibody, that interacts specifically with the target glycoprotein. Once separated from the sample, the target glycoprotein can be deglycosylated in situ, or eluted from the solid phase, quantitated, and then deglycosylated.