ISOLATION AND DEGLYCOSYLATION OF GLYCOPROTEINS
The invention provides more rapid and cost-effective methods of deglycosylating target glycoproteins. In methods of the invention, the target glycoprotein is isolated from initial samples, which may contain multiple other glycoproteins, by subjecting the initial sample to a solid phase containing an...
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Format | Patent |
Language | English French German |
Published |
09.04.2014
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Abstract | The invention provides more rapid and cost-effective methods of deglycosylating target glycoproteins. In methods of the invention, the target glycoprotein is isolated from initial samples, which may contain multiple other glycoproteins, by subjecting the initial sample to a solid phase containing an affinity ligand, such as a deglycosylated antibody, that interacts specifically with the target glycoprotein. Once separated from the sample, the target glycoprotein can be deglycosylated in situ, or eluted from the solid phase, quantitated, and then deglycosylated. |
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AbstractList | The invention provides more rapid and cost-effective methods of deglycosylating target glycoproteins. In methods of the invention, the target glycoprotein is isolated from initial samples, which may contain multiple other glycoproteins, by subjecting the initial sample to a solid phase containing an affinity ligand, such as a deglycosylated antibody, that interacts specifically with the target glycoprotein. Once separated from the sample, the target glycoprotein can be deglycosylated in situ, or eluted from the solid phase, quantitated, and then deglycosylated. |
Author | MURPHY, STEVEN M FULTON, SCOTT P |
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DocumentTitleAlternate | ISOLATION UND DEGLYKOSYLIERUNG VON GLYKOPROTEINEN ISOLATION ET DÉGLYCOSYLATION DES GLYCOPROTÉINES |
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RelatedCompanies | PROZYME, INC |
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Snippet | The invention provides more rapid and cost-effective methods of deglycosylating target glycoproteins. In methods of the invention, the target glycoprotein is... |
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Title | ISOLATION AND DEGLYCOSYLATION OF GLYCOPROTEINS |
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