Polynucleotide primers for detecting PIK3CA mutations

A polynucleotide comprising at least the final six nucleotides of one of the following primer sequences, or a sequence complementary thereto: SEQ. ID NOS. 10 to 16, 18 or 37 to 39. A method of detecting the presence or absence of the E545K mutation in the PIK3CA gene, preferably using an ARMS primer...

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Bibliographic Details
Main Authors BOARD, RUTH, FERGUSON, JENNIFER, RAVETTO, PAUL FRANCIS, THELWELL, NICOLA, WHITCOMBE, DAVID
Format Patent
LanguageEnglish
French
German
Published 03.10.2012
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Summary:A polynucleotide comprising at least the final six nucleotides of one of the following primer sequences, or a sequence complementary thereto: SEQ. ID NOS. 10 to 16, 18 or 37 to 39. A method of detecting the presence or absence of the E545K mutation in the PIK3CA gene, preferably using an ARMS primer combined with a Scorpion primer.
Bibliography:Application Number: EP20120169599