METHOD FOR WASHING CELLS

• Main Authors: YOSHIDA, KAZUHIRO, TSUCHIDA, HIROKI, SHANAHAN, ROBERT K, FORNACE, LUCAS V, KARASAWA, CHISATO
• Format: Patent
• Language: English; French; German
• Published: 05.10.2011
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL ORCHEMICAL PROCESSES; CENTRIFUGES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRANSPORTING; VINEGAR; WINE

A cell cluster from which unwanted components are removed by efficiently washing the cell cluster is collected. Provided is a cell washing method including a centrifuging step (S1) of collecting a cell suspension in a centrifuge container having a washing fluid discharge port opposing a bottom part and centrifugally separating cells at the bottom part by rotating in such a manner that the bottom part faces outward in the radial direction; a supernatant ejecting step (S2) of ejecting a supernatant centrifugally separated in the centrifuging step (S1); and a washing step (S3) of discharging the washing fluid from the discharge port while rotating the centrifuge container while ejecting the supernatant in the supernatant ejecting step (S2).