In vivo production of small interfering RNAs that mediate gene silencing

• Main Authors: MCLACHLAN, JUANITA, HUTVANGER, GYORGY, MELLO, CRAIG C, GRISHOK, ALLA, ZAMORE, PHILLIP D
• Format: Patent
• Language: English; French; German
• Published: 28.09.2016
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CARE OF BIRDS, FISHES, INSECTS; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW BREEDS OF ANIMALS; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; SUGARS; TRAPPING; VINEGAR; WINE

A method of making an engineered RNA precursor comprising the steps of: (a) selecting a sequence of a messenger RNA (mRNA) of a target gene of a mammalian cell; and (b) engineering an RNA precursor comprising; i) a first stem portion comprising a sequence of about 18 to about 40 nucleotides that is...