In vivo production of small interfering RNAs that mediate gene silencing
A method of making an engineered RNA precursor comprising the steps of: (a) selecting a sequence of a messenger RNA (mRNA) of a target gene of a mammalian cell; and (b) engineering an RNA precursor comprising; i) a first stem portion comprising a sequence of about 18 to about 40 nucleotides that is...
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Main Authors | , , , , |
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Format | Patent |
Language | English French German |
Published |
28.09.2016
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Abstract | A method of making an engineered RNA precursor comprising the steps of:
(a) selecting a sequence of a messenger RNA (mRNA) of a target gene of a mammalian cell; and
(b) engineering an RNA precursor comprising;
i) a first stem portion comprising a sequence of about 18 to about 40 nucleotides that is substantially complementary to the selected mRNA sequence;
ii) a second stem portion comprising a sequence of about 18 to about 40 nucleotides that is sufficiently complementary to the about 18 to about 40 nucleotide sequence of the first stem portion to hybridize with the first stem portion to form a duplex stem; and
iii) a loop portion that connects the two stem portions,
such that the engineered RNA precursor, when expressed in a cell, is processed by the cell to produce a duplex siRNA that mediates RNA interference (RNAi) by selectively cleaving the mRNA. |
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AbstractList | A method of making an engineered RNA precursor comprising the steps of:
(a) selecting a sequence of a messenger RNA (mRNA) of a target gene of a mammalian cell; and
(b) engineering an RNA precursor comprising;
i) a first stem portion comprising a sequence of about 18 to about 40 nucleotides that is substantially complementary to the selected mRNA sequence;
ii) a second stem portion comprising a sequence of about 18 to about 40 nucleotides that is sufficiently complementary to the about 18 to about 40 nucleotide sequence of the first stem portion to hybridize with the first stem portion to form a duplex stem; and
iii) a loop portion that connects the two stem portions,
such that the engineered RNA precursor, when expressed in a cell, is processed by the cell to produce a duplex siRNA that mediates RNA interference (RNAi) by selectively cleaving the mRNA. |
Author | MELLO, CRAIG C MCLACHLAN, JUANITA GRISHOK, ALLA ZAMORE, PHILLIP D HUTVANGER, GYORGY |
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DocumentTitleAlternate | Production in vivo de petits ARN interférents qui modèrent le silençage génique In-vivo-Herstellung kleiner interferierender und Genverstummung vermittelnder RNAs |
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Snippet | A method of making an engineered RNA precursor comprising the steps of:
(a) selecting a sequence of a messenger RNA (mRNA) of a target gene of a mammalian... |
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SubjectTerms | AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CARE OF BIRDS, FISHES, INSECTS CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW BREEDS OF ANIMALS NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS SUGARS TRAPPING VINEGAR WINE |
Title | In vivo production of small interfering RNAs that mediate gene silencing |
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