Method for purifying plasmid DNA having pharmaceutical quality
This invention provides a process for the continuous alkaline lysis of a bacterial suspension in order to harvest pDNA. It further provides for optional additional purification steps, including lysate filtration, anion exchange chromatography, triplex affinity chromatography, and hydrophobic interac...
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| Main Authors | , , , , |
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| Format | Patent |
| Language | English French German |
| Published |
03.11.2010
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| Subjects | |
| Online Access | Get full text |
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| Abstract | This invention provides a process for the continuous alkaline lysis of a bacterial suspension in order to harvest pDNA. It further provides for optional additional purification steps, including lysate filtration, anion exchange chromatography, triplex affinity chromatography, and hydrophobic interaction chromatography. These optional purification steps can be combined with the continuous lysis in order to produce a highly purified pDNA product substantially free of gDNA, RNA, protein, endotoxin, and other contaminants. |
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| AbstractList | This invention provides a process for the continuous alkaline lysis of a bacterial suspension in order to harvest pDNA. It further provides for optional additional purification steps, including lysate filtration, anion exchange chromatography, triplex affinity chromatography, and hydrophobic interaction chromatography. These optional purification steps can be combined with the continuous lysis in order to produce a highly purified pDNA product substantially free of gDNA, RNA, protein, endotoxin, and other contaminants. |
| Author | GUILLEMIN, THIERRY GAILLAC, DAVID COUDER, MICHEL BLANCHE, FRANCIS MAESTRALI, NICOLAS |
| Author_xml | – fullname: BLANCHE, FRANCIS – fullname: MAESTRALI, NICOLAS – fullname: COUDER, MICHEL – fullname: GUILLEMIN, THIERRY – fullname: GAILLAC, DAVID |
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| DocumentTitleAlternate | Verfahren zur Herstellung einer Plasmid-DNA in pharmazeutischer Qualität Procédé de préparation d'ADN plasmide de qualité pharmaceutique |
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| Notes | Application Number: EP20100157884 |
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| Snippet | This invention provides a process for the continuous alkaline lysis of a bacterial suspension in order to harvest pDNA. It further provides for optional... |
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| SubjectTerms | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| Title | Method for purifying plasmid DNA having pharmaceutical quality |
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