A METHOD OF PRODUCING GLUCAGON-LIKE PEPTIDE 1(GLP-1)7-36 AND AN GLP-1 ANALOGUE

• Main Authors: ZHOU, JIAXIANG, YU, GANG, ZHAO, SHAOLING, SUN, YUKUN, WU, DENGXI, WU, AIZHEN, ZHU, ZHIYONG
• Format: Patent
• Language: English; French; German
• Published: 14.04.2004
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

A genetically engineered bacterium for preparing the peptide GLP-1(7-36) promoting insulinotropic hormone secretion uses colibracillus as its host cell, which carries the plasmids containing 1-32 genes able to express GLP-1 (7-36). A genetically engineered bacterium for preparing the peptide GLP-1(7-36) promoting insulinotropic hormone secretion uses colibracillus as its host cell, which carries the plasmids containing 1-32 genes able to express GLP-1 (7-36). A process for configuring the bacterium includes cloning the 1-32 gene to colibacillus, transferring the plasmid to colibracillus, fermenting culture in liquid culture medium to generate and collect the inclusion body containing GLP-1(7-36), cracking fusion protein, separating and purifying. Its advantages include low cost.