Protein crystallography hanging drop lid that individually covers each of the wells in a microplate

• Main Authors: YOUNGBEAR, KATHY MARIE, SHA, MA
• Format: Patent
• Language: English; French; German
• Published: 28.01.2004
• Edition: 7
• Subjects: AFTER-TREATMENT OF SINGLE CRYSTALS OR A HOMOGENEOUSPOLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; APPARATUS THEREFOR; CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE; CHEMISTRY; CRYSTAL GROWTH; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PRODUCTION OF A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITHDEFINED STRUCTURE; REFINING BY ZONE-MELTING OF MATERIAL; SINGLE CRYSTALS OR HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITHDEFINED STRUCTURE; SINGLE-CRYSTAL-GROWTH; TRANSPORTING; UNIDIRECTIONAL SOLIDIFICATION OF EUTECTIC MATERIAL ORUNIDIRECTIONAL DEMIXING OF EUTECTOID MATERIAL

A lid that individually covers each of the wells in a microplate and methods for fabricating and using the lid are described herein. The lid includes a series of downwardly protruding necks each of which is sized to fit and seal against one of the wells formed within the microplate. And, each neck has a surface covered with a rubber-like substance that ensures a relatively tight seal with each well. In operation, the lid and microplate are preferably used together to grow protein crystals using a hanging drop vapor diffusion crystallization process.