Cloning and expression of genes encoding arabinan-degrading enzymes of fungal origin

• Main Authors: ANDREOLI, PETER MICHAEL, VAN HEUVEL, MARGARETHA, VISSER, JACOB, BAKHUIS, JANNA GARDINA, HARDER, ABRAHAM, DE GRAAFF, LEENDERT HENDRIK, VAN DER VEEN, PETER, FLIPPHI, MICHEL JOHANNES ANTHONIE, COUTEL, YVES
• Format: Patent
• Language: English; French; German
• Published: 14.11.2001
• Edition: 7
• Subjects: APPARATUS THEREFOR; BEER; BIOCHEMISTRY; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FODDER; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PAPER-MAKING; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT,VEGETABLES, EDIBLE SEEDS; PROCESSES USING MICROORGANISMS; PRODUCTION OF CELLULOSE; PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCESFROM CELLULOSE-CONTAINING MATERIALS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REGENERATION OF PULPING LIQUORS; SPIRITS; TEXTILES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE

The present invention provides the cloning and expression of purified and isolated DNA molecules, obtainable from fungi, which encode enzymes having arabinan-degrading activity. The present invention also provides DNA constructs containing these DNA molecules and methods for their enhanced expression in selected microbial host cells. The present invention further provides methods for the use of the thus-produced arabinan-degrading enzymes.