Exonuclease mediated strand displacement amplification

This invention relates a nucleic acid target amplification method which operates at a single temperature and makes use of a polymerase in conjunction with an exonuclease that will digest the primer portion of the polymerized strand such that the polymerase will displace the partially digested strand...

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Bibliographic Details
Main Authors SCHRAM, JAMES L, WALKER, GEORGE T
Format Patent
LanguageEnglish
French
German
Published 26.08.1992
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:This invention relates a nucleic acid target amplification method which operates at a single temperature and makes use of a polymerase in conjunction with an exonuclease that will digest the primer portion of the polymerized strand such that the polymerase will displace the partially digested strand without digestion of the target strand while generating a newly polymerized strand.
Bibliography:Application Number: EP19920300752