Nucleotide sequence coding for an outer membrane protein from Neisseria meningitidis and use of said protein in vaccine preparations

• Main Authors: ALVAREZ ACOSTA, ANABEL, GRILLO, JUAN MORALES, CRUZ LEON, SILIAN, FERNANDEZ MASO, JULIO RAUL, NIETO, GERARDO GUILLEN, GONZALEZ BLANCO, SONIA, HOUSSEIN SOSA, MANUEL SELMAN, MORERA CORDOVA, VIVIAN, SANTOS, BEATRIZ TAMARGO, MENENDEZ, EVELIN CABALLERO, DEL VALLE ROSALES, JESUS AUGUSTO, COUZEAU RODRIGUEZ, EDELGIS, HERRERA MARTINEZ, LUIS S. CENTRO INGENIERIA, RODRIGUEZ, RICARDO SILVA, NOVOA PEREZ, LIDIA INES, MUSACCHIO LASA, ALEXIS
• Format: Patent
• Language: English; French; German
• Published: 11.03.1992
• Edition: 5
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The present invention is concerned with a method for the isolation of a nucleotide sequence which codes for a protein having a molecular weight of about 64 000 daltons, which is located on the outer membrane of N. meningitidis, as well as with the recombinant DNA obtained therefrom, which is used for the transformation of a host microorganism. The technical object pursued with the invention is the identification of a nucleotide sequence coding for a highly conserved and common protein for the majority of pathogenic Neisseria strains, the production of this protein with a high level of purity and in commercially useful amounts using the recombinant way, so that it can be used in diagnostic methods and vaccine preparations with a broad immunoprotection spectrum.