Cloning and expression of xylanase genes from fungal origin

• Main Authors: VISSER, JACOB, VAN DEN BROECK, HENRIETTE CATHARINA, DE GRAAFF, LEENDERT HENDRIK, HARDER, ABRAHAM, HILLE, JAN DIRK RENE, VAN OOYEN, ALBERT JOHANNES JOSEPH
• Format: Patent
• Language: English; French; German
• Published: 27.01.2010
• Subjects: APPARATUS THEREFOR; BAKERY PRODUCTS; BAKING; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; EDIBLE DOUGHS; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FODDER; FOODS OR FOODSTUFFS; HUMAN NECESSITIES; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PAPER-MAKING; PRESERVATION THEREOF; PROCESSES USING MICROORGANISMS; PRODUCTION OF CELLULOSE; PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCESFROM CELLULOSE-CONTAINING MATERIALS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REGENERATION OF PULPING LIQUORS; SPIRITS; TEXTILES; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BYADDITION OF MATERIALS; VINEGAR; WINE

Methods and expression constructs are provided for the cloning of genes from fungal origin which encode polypeptides having xylanase activity and their overexpression in a selected microbial host cell. Xylanases of fungal origin generally have lower pH optima and remain stable over a broader pH range than do xylanases of bacterial origin. The present invention provides for the high level production of fungal xylanases which are may be used in a variety of industrial applications requiring xylanase activity at a low pH.