Verfahren zur enzymatischen Galactosylierung von Mono- und Oligosacchariden

• Main Authors: MARQUARDT, RUEDIGER., 60389 FRANKFURT, DE, ZERVOSEN, ASTRID., WELKENRAEDT, BE, ELLING, LOTHAR., 52066 AACHEN, DE, HOERSCH, BRIGITTE., 65830 KRIFTEL, DE, KULA, MARIA-REGINA, DR., 52382 NIEDERZIER-HAMBACH, DE, SEIFFERT-STOERIKO, ANDREAS., 65929 FRANKFURT, DE
• Format: Patent
• Language: German
• Published: 14.11.1996
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; DERIVATIVES THEREOF; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; SPIRITS; SUGARS; VINEGAR; WINE

The invention concerns an improved method for the enzymatic galactosylation of monosaccharides and oligosaccharides with in situ regeneration of the nucleotide sugar (or the nucleoside diphosphate sugar) in the presence of saccharose synthase, beta -1-4-galactosyl transferase and uridine diphosphate glucose-4'-epimerase (UDP glucose-4'-epimerase), the method calling for the uridine diphosphate glucose-4'-epimerase to be reactivated by a ketone sugar derivative.