VERFAHREN ZUR REINIGUNG VON NUCLEO IDSEQUENZEN
A method for obtaining a strain of microorganisms modified to contain a fragment of a specific deoxyribonucleotide sequence, which method comprises: (I) purifying a fragment of a deoxyribonucleotide sequence by the following steps: (a) providing a population of cdna transcripts of the polyribonucleo...
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Main Authors | , , |
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Format | Patent |
Language | German |
Published |
14.01.1981
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Edition | 3 |
Subjects | |
Online Access | Get full text |
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Abstract | A method for obtaining a strain of microorganisms modified to contain a fragment of a specific deoxyribonucleotide sequence, which method comprises: (I) purifying a fragment of a deoxyribonucleotide sequence by the following steps: (a) providing a population of cdna transcripts of the polyribonucleotides. (b) submit the cdna transcripts to the action of a restriction endonuclease preparation capable of catalyzing the hydrolysis of the cdna transcripts at each of the restriction sites. (c) fractionating the fragments produced by the action of the restriction endonuclease according to its length. (d) pretreating the dna to remove any 5''-phosphate terminal group. (e) incubating the dna with a restriction endonucelase capable of acting on the desired specific nucleotide sequence to produce two linear sub-fragments thereof. (f) fractionating the sub-fragments according to their length. (g) isolate the two sub-fragments, (h) assemble the sub-fragments covalently, in a reaction catalyzed by dna ligase, (I) fractionate the assembled dna molecules according to their length, (II) recombine the resulting product of the above sequence with a dna transfer vector and transfer it to a microorganism. (Machine-translation by Google Translate, not legally binding) |
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AbstractList | A method for obtaining a strain of microorganisms modified to contain a fragment of a specific deoxyribonucleotide sequence, which method comprises: (I) purifying a fragment of a deoxyribonucleotide sequence by the following steps: (a) providing a population of cdna transcripts of the polyribonucleotides. (b) submit the cdna transcripts to the action of a restriction endonuclease preparation capable of catalyzing the hydrolysis of the cdna transcripts at each of the restriction sites. (c) fractionating the fragments produced by the action of the restriction endonuclease according to its length. (d) pretreating the dna to remove any 5''-phosphate terminal group. (e) incubating the dna with a restriction endonucelase capable of acting on the desired specific nucleotide sequence to produce two linear sub-fragments thereof. (f) fractionating the sub-fragments according to their length. (g) isolate the two sub-fragments, (h) assemble the sub-fragments covalently, in a reaction catalyzed by dna ligase, (I) fractionate the assembled dna molecules according to their length, (II) recombine the resulting product of the above sequence with a dna transfer vector and transfer it to a microorganism. (Machine-translation by Google Translate, not legally binding) |
Author | SHINE,JOHN,AU SEEBURG,PETER H.,DE GOODMAN,HOWARD M.,US |
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SubjectTerms | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE |
Title | VERFAHREN ZUR REINIGUNG VON NUCLEO IDSEQUENZEN |
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