STRAIN OF MICROORGANISM BREVIBACTERIUM FLAVUM CCM 4125

• Main Authors: HANO ALEXANDER,CS, PLACHY JIRI RNCSC.,CS, PILAT PETR CSC.,CS, BUCKO MICHAL DRSC.,CS, ULBERT STANISLAV ,CS, CULIK KAREL RNDR.,CS, BOROSOVA GABRIELA ,CS
• Format: Patent
• Language: English
• Published: 12.11.1991
• Edition: 5
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The purpose of the solution consists in production of essential aminoacid of lysine, used mainly as a fodder additive. Lysine is synthesized and accumulated in medium by mutant of bacterial strand Brevibacterium flavum, isolated after induction by mutagen using selective media. The mutant, resistant against S-(2-aminoethyl)-L-cysteine, dependant on homoserine, leucine and alanine, in which phagoresistance as well as sensitivity towards fluorpyruvate was detected, cultivated in medium with saccharose as the source of carbon, hydrolysate of peanut flour and corn-steep liquor as the source of organic nitrogen, and ammonium sulphate as the source of inorganic nitrogen and with mineral salts cumulated during conversion 40 percent in aerobic conditions at the temperature of 28 degrees C and neutral pH in the environment 60g/L of L-lysine. The lysine can be produced in this manner using standard technological procedures.