Method, capture probe and system suitable for specifically extracting target nucleic acid from large-volume sample

• Main Authors: YE DINGWEI, JU JINLIANG, WU, JUINERONG
• Format: Patent
• Language: Chinese; English
• Published: 23.08.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a method, a capture probe and a system suitable for specifically extracting target nucleic acid from a large-volume sample, and belongs to the technical field of nucleic acid extraction. According to the method provided by the invention, a plurality of specific capture nucleic acids C which are labeled by a marker B and can be specifically bound with target nucleic acids and a solid-phase carrier carrying a marker A capable of being specifically bound with the marker B are utilized, and the target nucleic acids in a sample are bound to the solid-phase carrier through the capture nucleic acids C to form a solid-phase carrier-A-B-C-target nucleic acid compound; furthermore, the target nucleic acid in a large-volume sample can be specifically captured and extracted. The invention also provides a capture probe and a system suitable for specifically extracting PCA3 and SPDEF mRNA from a large-volume sample. 本发明公开一种适用于从大体积样本中特异性提取靶核酸的方法、捕获探针和体系，属于核酸提取的技术领域。本发明提供的方法利用多个被标记物B标记的能够与靶核酸特异性结合的特定捕