ShRNA (short hairpin ribonucleic acid) adeno-associated virus vector as well as construction method and application thereof

• Main Authors: CHEN WEI, DU GUANGYI, ZHANG MING, XIE LIANGHUA
• Format: Patent
• Language: Chinese; English
• Published: 30.07.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention provides a shRNA (short hairpin Ribonucleic Acid) adeno-associated virus vector as well as a construction method and application thereof, and belongs to the technical field of biological medicines. The invention provides shRNA for targeted inhibition of ARG1 expression, a shuttle plasmid, a recombinant adeno-associated virus vector and a recombinant adeno-associated virus are constructed based on the shRNA, the recombinant adeno-associated virus (AAV) is used as the vector and carries an ARG1-shRNA sequence, and the ARG1-shRNA interference plasmid can significantly and specifically inhibit the ARG1 expression, so that the ARG1 expression can be inhibited, and the ARG1 expression can be inhibited. The polypeptide has the effect of resisting vascular endothelial dysfunction induced by the type 2 diabetes, is high in safety and provides a novel ARG1 targeting treatment strategy for cardiovascular diseases of the type 2 diabetes. 本发明提供了一种shRNA腺相关病毒载体及其构建方法和应用，属于生物医药技术领域。本发明提供了一种靶向抑制ARG1表达的shRNA，并基于所