Method for detecting multiple cytokines of memory immune cells

• Main Authors: ZHANG LING, PAN JIANHUA, ZHOU RUIXIONG, XIANG CHENYAN, HU YAN
• Format: Patent
• Language: Chinese; English
• Published: 26.07.2024
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention belongs to the technical field of medical detection, and discloses a method for detecting multiple cytokines of memory immune cells, which comprises the following steps: performing stimulation treatment on cell suspension of peripheral blood mononuclear cells by using antigen stimulating peptides for stimulating memory T cells, and setting negative control treatment and positive control treatment; detecting the treated peripheral blood mononuclear cells by adopting a FluroSpot human interferon gamma/interleukin 2/tumor necrosis factor kit; wherein the ratio of the peripheral blood mononuclear cells to the antigen stimulating peptide is (240000-260000): 0.1 [mu] L to 0.5 [mu] L, and the concentration of the antigen stimulating peptide is 1.5 [mu] g/mL to 4 [mu] g/mL. The method for detecting the multiple cytokines of the memory immune cells can improve the fluorescent quantitative detection effect on various cytokines generated by the memory T cells. 本发明属于医学检测技术领域，公开了一种检测记忆性免疫细胞多种细胞因子的方法，包括如下步骤：将