Liquid chromatography-mass spectrometry detection method for improving chromatographic behavior of oligonucleotide drug

• Main Authors: LU JINLIAN, AN PEIYUN, XING LILI, SHEN LIANG, WU XIAOPENG, TAO YI
• Format: Patent
• Language: Chinese; English
• Published: 19.07.2024
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a liquid chromatography-mass spectrometry detection method for improving the chromatographic behavior of an oligonucleotide drug, a pretreatment solution is added in the pretreatment process, a sample is pretreated by combining solid-phase extraction and other pretreatment steps, liquid chromatography-mass spectrometry detection is completed, and the pretreatment solution is composed of animal waste and waste homogenate. According to the detection method provided by the invention, through the steps of preparing and adding the pretreatment liquid, the influence caused by adsorption is effectively reduced, the peak shape is symmetrical, and a wider linear range can be obtained, so that the sample detection requirements under different doses are met, the defects of poor linearity, chromatographic peak trailing and large residue in the prior art are overcome, and the detection method is suitable for industrial production. In the peak shape obtained through detection, the trailing phenomeno