Construction method and application of WNT1 mutant gene knock-in rat osteogenesis imperfect animal model

• Main Authors: XU HONGWEN, WANG XIUMIN, TANG YAPING, SHANG LIYUAN, ZHU MINGWEI, LI XIN, SHI WEIZHE, XU YIBO, NONG TIANYING, LI XIA, XIAN CAIXIA
• Format: Patent
• Language: Chinese; English
• Published: 21.06.2024
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CARE OF BIRDS, FISHES, INSECTS; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW BREEDS OF ANIMALS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; SPIRITS; TRAPPING; VINEGAR; WINE

The invention belongs to the technical field of biomedicine, discloses a construction method and application of a WNT1 mutant gene knock-in rat osteogenesis imperfect animal model, and particularly discloses sgRNA of a specific target WNT1 mutant gene, and the sequence of the sgRNA is GCCGATGGTGGTAA...