Method for processing cells

• Main Authors: HARADA YUI, YONEMITSU YOSHIKAZU
• Format: Patent
• Language: Chinese; English
• Published: 28.05.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The present invention provides a method for thawing frozen highly active NK cells while maintaining high activity. The liquid for suspending the high-activity NK cells, which meets the following conditions, is used for unfreezing the frozen cells. Provided is a liquid for suspending cells administered to a human, which satisfies the following conditions. (1) contains potassium ions, (2) has a pH of 6.4 or more, (3) does not contain chloride ions at a concentration of 135 mEq/L or more, (4) does not contain calcium ions at a concentration of 0.423 mM or more, and (5) has an osmotic pressure of 200-396 mOsm. 本发明提供了在维持高活性的情况下对冷冻的高活性NK细胞进行解冻的方法。将满足以下条件的用于悬浮高活性NK细胞的液体用于冷冻的细胞的解冻。提供了用于悬浮对人施用的细胞的液体，其满足以下条件。(1)含有钾离子，(2)pH为6.4以上，(3)不含有135mEq/L以上的浓度的氯离子，(4)不含有0.423mM以上的浓度的钙离子，(5)渗透压为200-396mOsm。