In situ epitranscriptome analysis

• Main Authors: WANG XIAO, TIAN, JILEI, ZENG HONGBO, REN JIANGTAO, GUO JIMING
• Format: Patent
• Language: Chinese; English
• Published: 24.05.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The present disclosure provides methods, compositions, and systems for analyzing epitranscriptome RNA modifications in a cell. The present disclosure also provides methods for analyzing the interaction between one or more RNAs of interest and RNA binding proteins in a cell (e.g., introducing epitranscriptome modified proteins on RNAs of interest). The disclosure also provides methods for diagnosing a disease or condition in a subject based on the analysis of epitranscriptome RNA modifications in cells, including cells within intact tissue, or the analysis of interactions between RNA binding proteins and RNA. The present disclosure also provides methods of screening or testing candidate agents capable of modulating epitranscriptome modifications of one or more RNAs or interactions between one or more RNAs and RNA binding proteins. The disclosure also provides methods for treating a disease or condition in a subject in need thereof. The present disclosure also describes probe pairs and probe sets comprising oli